Plant tissue culture (PTC) is a biotechnological technique used to grow plant cells, tissues, or organs in an aseptic environment on a synthetic nutrient medium. It relies on totipotency, the inherent ability of a single plant cell to regenerate into a whole, functional plant. Foundational Concepts
Callus Culture: The growth of an undifferentiated mass of cells. It is often the first step in generating genetic variation (somaclonal variation). plant tissue culture ppt pdf
Further Reading & References (To add to your PDF): Plant tissue culture (PTC) is a biotechnological technique
Step 2: Surface Sterilization The explant must be sterilized to remove surface microbes. Automated micropropagation and robotics